In a clip flagged by Right Wing Watch, the son of renowned pastor Rev. Billy Graham spoke to Family Research Council head Tony Perkins in a radio interview.
"His mother must have been a Muslim," Graham said. "We don't know that, but she married two Muslim men, so there must have been something there."
"And the framework that the President has growing up, his influences in his life, was that of Islam," he added.
According to Obama's maternal sister, their mother Ann Dunham was "an agnostic." In his book "The Audacity of Hope," Obama wrote that his mother exposed him to all kinds of different religious practices.
"In our household the Bible, the Koran, and the Bhagavad Gita sat on the shelf alongside books of Greek and Norse and African mythology," he wrote.
Graham also claimed that Obama's father was "a Muslim." (Obama has said that his father was born to a Muslim family but became an atheist.)
The reverend said that like many Americans, he enjoyed a Christian upbringing, unlike Obama.
"Our President did not have that. It was Islam. And many feel that he's protecting Islam," he said. "I don't know that, but it certainly seems that way."
Listen to the clip: